Journal: Breast Cancer Research : BCR

Article Title: BHLHE22, targeted by deubiquitinating enzyme OTUD3, exerts an antitumor role in triple-negative breast cancer progression via transcriptionally inhibiting CDT1

doi: 10.1186/s13058-025-02198-0

Figure Lengend Snippet: mRNA-seq analysis identifies downstream target genes of BHLHE22. a , b The results of 2D and 3D principal component analysis (PCA) of the sequenced samples. c Taking |log2(fold change)|≥ 2 and p value < 0.001 as thresholds, the volcano map showed the differential expression of genes in BHLHE22-overexpressed cells. d The Cluego network diagram showed that the enriched functions of these differentially expressed gene (DEGs). e KEGG enrichment analysis of DEGs was shown by bubble map. f The link and overlapping of associated molecules among proliferation-related terms were presented as Cluego network diagram. g The Venn diagram showed overlapping genes in these four terms. h The expression of overlapping genes in BHLHE22-overexpressed cells and control cells was shown with heat maps

Article Snippet: DNA replication in the cells was detected using the EdU cell proliferation assay kit (cat. E-CK-A370, Elabscience, China).

Techniques: Quantitative Proteomics, Expressing, Control